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The next August Journal Club will be on Tuesday August 29th at 13:00 EST/18:00 UK and will be streamed live from Cooper Union to our Facebook page.

We have been talking about a number of new studies in past Journal Clubs, so we thought it was time to take a look at the actual delivery methods and the hurdles we face in the field to develop effective therapies.

We will be discussing the delivery methods for gene therapies and in particular this recent paper:

Gallego-Perez, D., Pal, D., Ghatak, S., Malkoc, V., Higuita-Castro, N., Gnyawali, S., … & Singh, K. (2017). Topical tissue nano-transfection mediates non-viral stroma reprogramming and rescue. Nature nanotechnology.

CategoryJournal club
About the author

Oliver Medvedik

Oliver Medvedik, Co-founder of Genspace citizen science laboratory in Brooklyn NY, earned his Ph.D. at Harvard Medical School in the Biomedical and Biological Sciences program. As part of his doctoral work he has used single-celled budding yeast as a model system to map the genetic pathways that underlie the processes of aging in more complex organisms, such as humans. Prior to arriving in Boston for his doctoral studies, he has lived most of his life in New York City. He obtained his bachelor’s degree in biology from Hunter College, City University of New York. Since graduating from Harvard, he has worked as a biotechnology consultant, taught molecular biology to numerous undergraduates at Harvard University and mentored two of Harvard’s teams for the international genetically engineered machines competition (IGEM) held annually at M.I.T.
  1. August 27, 2017

    It would be cool if there could be some discussion on megaTALs and whether or not they are the answer gene editing is looking for?

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3936731/

    “MegaTALs exhibit dramatic increases in cleavage activity compared with their isolated mn cleavage heads, an effect that appears to be an outcome of the auxiliary TAL effector DNA-binding domain docking (‘addressing’) the mn adjacent to its target and effectively increasing the rate of enzyme–substrate complex formation. Furthermore, the mn-mediated cleavage produces DNA ends with 3′ overhangs that are efficiently processed by the Trex2 exonuclease, thus allowing the co-expression of a megaTAL and Trex2 to achieve unprecedentedly high targeted modification rates in primary cells of >70%.”

  2. August 27, 2017

    Also could the recently developed technology of Spherical Nucleic Acids delivering whole enzymes to cells be useful?

    DNA-Mediated Cellular Delivery of Functional Enzymes:

    https://pubs.acs.org/doi/abs/10.1021/jacs.5b09711

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